个人简述:

男，1975年生，副研究员，硕士研究生导师，生物化学与分子生物学学科主任，中国生物化学与分子生物学会分子生物技术专业委员会委员。2004年7月江南大学硕士研究生毕业，同年进入浙江理工大学生命科学学院工作。从事家蚕生物反应器生物制药与家蚕功能基因组学的研究。主要开展家蚕非编码RNA及部分生长发育相关基因的研究工作，同时开展家蚕生物反应器制备疫苗和抗菌肽的研究工作。2009年破格评为硕士研究生导师，2010年破格晋升为副研究员。发表论文50余篇，其中SCI收录30余篇，主编专著1本

科研工作:

获奖情况

1、“rhGM-CSF口服生物利用度研究：单剂量随机开放双交叉的临床试验”获浙江省自然科学学术奖二等奖；

2、“数学方法在生物序列分析中的应用”获得浙江省高校优秀科研成果二等奖；

3、“家蚕表达的双联治疗前列腺疾病新药的研制”获天津市滨海新区科学技术奖；

科研项目

1. 家蚕关键品质性状分子解析及分子育种基础研究，国家973项目子课题，2012.1-2016.12，684万元，总课题骨干，个人主持经费80万元

2. 家蚕bmo-miR-7和miR-79靶基因的筛选及调控Notch途径靶基因的鉴定，浙江省自然科学基金，2012.1-2013.12，8万元，主持

3. 柞蚕杆状病毒(AnpeNPV)Bacmid表达系统的构建，国家自然科学基金，2008.1-2008.12，10万元，主持

4. 家蚕生物反应器产业化体系与产品开发，国家863重点项目，836万元，2011.6-2014.12排名12

5. 基于多源信息融合的蛋白质亚细胞定位预测算法研究，国家自然科学基金，2012.1-2015.12，80万元，排名3

6. 家蚕microRNA基因功能鉴定与功能研究，国家自然科学基金，2007.1-2009.12，25万元，排名3

7. 家蚕小分子RNA、小肽功能以及病原体与宿主相互作用机理研究，国家973项目，2005.9-2010.12，232.8万元，排名9

8. 家蚕和昆虫蛹生物反应器国家，国家863项目，2008.01-2010.12，170万元，排名5

主要论文

1.Wu C, Wang C, Li D, Liu Y, Sheng Q, Lv Z, Yu W, Nie Z. BmHSP20.8 is Localized in the Mitochondria and has a Molecular Chaperone Function In Vitro. J Insect Sci, 2015, 15. pii: 99. (通讯作者)

2.Nie Z, Zhu H, Zhou Y, Wu C, Liu Y, Sheng Q, Lv Z, Zhang W, Yu W, Jiang C, Xie L, Zhang Y, Yao J. Comprehensive profiling of lysine acetylation suggests the widespread function is regulated by protein acetylation in the silkworm, Bombyx mori. Proteomics, 2015, 15(18):3253-66.

3.Min Liu, Chan Wang, Dan Li, Yue Liu, Qing Sheng, Zhengbing Lv, Wei Yu, Dan Wang, Yaozhou Zhang, Zuoming Nie. Cloning and Expression Characteristics of the Notch-Associated Gene BmE(spl)mgama from Silkworm, Bombyx mori. Appl Biochem Biotechnol, 2014, 173(8):2065-75. (通讯作者)

4.Nie Z, Zhou F, Li D, Lv Z, Chen J, Liu Y, Shu J, Sheng Q, Yu W, Zhang W, Jiang C, Yao Y, Yao J, Jin Y, Zhang Y. RIP-seq of BmAgo2-associated small RNAs reveal various types of small non-coding RNAs in the silkworm, Bombyx mori. BMC Genomics, 2013, 14:661

5.Zhou F, Gao Z, Lv Z, Chen J, Hong Y, Yu W, Wang D, Jiang C, Wu X, Zhang Y, Nie Z. Construction of the ie1-Bacmid Expression System and Its Use to Express EGFP and BmAGO2 in BmN Cells. Appl Biochem Biotechnol, 2013, 169(8):2237-47. (通讯作者)

6.Yang L, Lu X, Liu Y, Lv Z, Chen J, Yu W, Zhang Y, Nie Z. Expression analysis of miRNAs in BmN cells. Gene, 2012, 505(2):240-245(通讯作者)

7.Nie Z, Lv Z, Qian J, Chen J, Li S, Sheng Q, Wang D, Shen H, Yu W, Wu X, Zhang Y. Molecular cloning and expression characterization of translationally controlled tumor protein in silkworm pupae. Mol Biol Rep, 2010, 37(6):2621-2628

8.Zuoming Nie, Ruobing Zhou, Jian Chen, DanWang, Zhengbing Lv, Pingan He, Xuedong Wang, Hongdan Shen, Xiangfu Wu, Yaozhou Zhang. Subcellular localization and RNA interference of an RNA methyltransferase gene from silkworm, Bombyx mori. Comparative and functional genomics, 2008:571023.

9.Zuoming Nie, Jiangtao Xu, Jian Chen , Zhengbing Lv, Dan Wang, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression Analysis and Characteristics of Profilin Gene from Silkworm, Bombyx mori. Applied Biochemistry and Biotechnology, 2009,158 (1):59-71.

10.Ping-an He*, Zuoming Nie*, Jianqing Chen, Jian Chen, Zhengbing Lv, Qing Sheng, Songping Zhou, Xiaolian Gao, Lingyin Kong, Xiangfu Wu, Yongfeng Jin, Yaozhou Zhang. Identification and characteristics of microRNAs from Bombyx mori. BMC:Genomics, 2008, 9:248. (并列第一作者).

11.Nie ZM, Zhang ZF, Wang D, He PA, Jiang CY, Song L, Chen F, Xu J, Yang L, Yu LL, Chen J, Lv ZB, Lu JJ, Wu XF, Zhang YZ. Complete sequence and organization of Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics. 2007, 8:248.

12.Li Y, Wang W, Cheng D, Wang T, Lu C, Chen J, Nie Z, Zhang W, Lv Z, Wu W, Shu J. A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. Mar Drugs, 2015, 13(5):2955-66.

13.Zhang W, Wang J, Zhang C, Fang Q, Shu J, Li S, Jin J, Wang D, Nie Z, Lv Z, Zhang Y. Synergetic Protein Factors That Improve rhGM-CSF Absorption via an Oral Route Exist in Silkworm Pupae. Mol Pharm, 2015, 12(5):1347-55.

14.Chen J, Shu T, Chen J, Ye M, Lv Z, Nie Z, Gai Q, Yu W, Zhang Y. Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori. Protein J, 2015, 34(4):256-66.

15.Wenping Zhang, Changrong Zhang, Zhengbing Lv, Dailing Fang, Dan Wang, Zuoming Nie,Wei Yu, Hanglian Lan, Caiying Jiang, Yaozhou Zhang. Molecular Characterization, Tissue Distribution,Subcellular Localization and Actin-Sequestering Function of a Thymosin Protein from Silkworm. PLoS ONE 2012,7(2),e31040

16.Wenping Zhang, Hanglian Lan, Tao Wu, Caiying Jiang, Zhengbing Lv,Zuoming Nie, Xiangfu Wu ,Yaozhou Zhang. Expression Analysis and Characteristics of Hypothetical Protein BmLOC778500 from Silkworm, Bombyx mori African Journal of Biotechnology 2011,10(19) :3753-3761.

17.Chen J, Gai Q, Lv Z, Chen J, Nie Z, Wu X, Zhang Y.All-trans retinoic acid affects subcellular localization of a novel BmNIF3l protein: functional deduce and tissue distribution of NIF3l gene from silkworm (Bombyx mori). Arch Insect Biochem Physiol. 2010, 74(4):217-31.

18.Hanglian Lan, Zuoming Nie, Yue Liu, Zhengbing Lv, Yingshuo Liu, Yanping Quan, Jianqing Chen, Qingliang Zhen, Qin Chen, Dan Wang, Qing Sheng, Wei Yu, Jian Chen, Xiangfu Wu, and Yaozhou Zhang. In vivo Bioassay of Recombinant human growth hormone synthesized in B. mori pupae. Journal of Biomedicine and Biotechnology, 2010:306462.

19.Sheng Q, Xia J, Nie Z, Zhang Y*. Cloning, Expression, and Cell Localization of a Novel Small Heat Shock Protein Gene: BmHSP25.4.Appl Biochem Biotechnol, 2010 Jan 14. 2010, 162(5):1297-305.

20.Zeng F, Xie H, Nie Z, Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang Y. Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori. Comp Funct Genomics, 2009:947490.

21.Shen Y, Zhang T, Chen J, Lv Z, Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q, Wu X, Zhang Y*. Molecular Characterization and Tissue Localization of an F-Box Only Protein from Silkworm, Bombyx mori. Comp Funct Genomics. 2009:416040.

22.Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics, 2009, 9(4):447-54.

23.Zhang W, Lv Z, Nie Z, Chen G, Chen J, Sheng Q, Yu W, Jin Y, Wu X, Zhang Y. Bioavailability of orally administered rhGM-CSF: a single-dose, randomized, open-label, two-period crossover trial. PLoS One. 20094(5):e5353.

24.Yaozhou Zhang, Jian Chen, Zhengbing Lv, Qin Chen, Yanping Quan, Lingyin Kong, Haihua Zhang, Si Li, Qingliang Zheng, Jianqing Chen, Zuoming Nie, Jiang Wang, Yongfeng Jin, Xiangfu Wu. 2008. A Novel Method for Isolation of Membrane Proteins: A Baculovirus Surface Display (BVSD) System. Proteomics, 8(20): 4178-4185.

25.Yao YH, Dai Q, Nan XY, He PA, Nie ZM, Zhou SP, Zhang YZ. Analysis of similarity/dissimilarity of DNA sequences based on a class of 2D graphical representation. J Comput Chem, 2008, 29(10):1632-1639.

26.Chen J, Nie ZM, Lv ZB, Zhu CG, Xu CZ, Jin YF, Wu XF, Zhang YZ. Large-Scale Purification of hGM-CSF Expressed in Bombyx mori Pupae. Applied Biochemistry and Biotechnology. 2007, 141(1):149-160.

27.Zhang YZ, Chen J, Nie ZM, Lu ZB, Wang D, Jiang CY, He PA, Liu LL, Lou YL, Song L, Wu XF. Expression of open reading frames in silkworm pupal cDNA library. Applied Biochemistry and Biotechnology. 2007, 136(3):327-343.

28.Qingliang Zheng, Jian Chen, Zuoming Nie, Zhengbing Lv, Dan Wang, Yaozhou Zhang. Expression, purification and characterization of a three-domain Kazal-type inhibitor from silkworm pupae (Bombyx mori). Comp Biochem Physiol B Biochem M Biol, 2007, 146: 234-240.

29.Xuejian Wang, Jian Chen, Zhengbing Lv, Zuoming Nie, Dan Wang, Hongdan Shen, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression and Functional Analysis of The Cellular Retinoic Acid Binding Protein From Silkworm Pupae (Bombyx mori). Journal of Cellular Biochemistry, 2007, 102（4）:970-979.

30.Lingyin Kong, Zhengbing Lv, Jian Chen, Zuoming Nie, Dan Wang, Hongdan Shen, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori). BBA-General subject, 2007,1770(12): 1598-604

31.Jianqing Chen, Jian Chen, Qijing Gai, Zhengbing Lv, Dan Wang, Zuoming Nie, Jiang Wang, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Molecular Characterization and Immunohistochemical Localization of a Novel Troponin C During Silkworm Development. Cell and tissue research, 2008,331(3): 725-38

32.Yaozhou Zhang, Jian Chen, Zhengbing Lv, Zuoming Nie, Xiaoyan Zhang, XiangfuWu. Can 29kD rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through Orally Administration?. European. Joural of Pharmaceutical Sciences,2006,28(3):212-223

33.Wei Yu, Jian Chen, Xiuling Zhao, Zhengbing Lv, Zuoming Nie, Yaozhou Zhang.Expression of polyhedrin-hEGF fusion protein incultured cells and larvae of Bombyx mori. African Journal of Biotechnology,2006,5 (11): 1034-1040.