科研工作:

1、国家科技支撑计划项目，奶牛主要呼吸道和消化道传染病快速检测、疫苗及防控技术研究（2012BAD12B03-3），2012-20162、黑龙江省科技计划项目，猪重要传染病快速诊断试剂盒及口服基因工程疫苗的研究，2005-2009。近五年代表性著作、论文： 1.GuanWeikun,LiuWenxin,BaoJun,LiJinping,YuanChaowen,TangJie,ShiDongfang.AnalysisandapplicationofaneutralizinglinearepitopeonliabletoxinBofenterotoxinEscherichiacoli..Appliedmicrobiologyandbiotechnology.2015Jul99(14):5985-96.2.WenxinLiu,XingyueLi,JunBao,WeikunGuan,ZhitengZhao,ChaowenYuan,JieTang,DongfangShi.Constructionandevaluationofafluorescence-basedliveattenuatedEscherichiacolideliverysystemforgeneratingoralvaccinecandidate.ApplMicrobiolBiotechnol,2015,99(9):4005-4018.DOI10.1007/s00253-014-6332-0.3.WenxinLiu,JinpingLi,JunBao,XingyueLi,WeikunGuan,ChaowenYuan,JieTang,ZhitengZhao,DongfangShi.2014.SimultaneousoralimmunizationofmicewithliveattenuatedEscherichiacoliexpressingLT192-STa13andLT192-STbfusionimmunogen,respectively,forpolyvalentvaccinecandidate.ApplMicrobiolBiotechnol,2015,99(9):3981-3992.(3.811),DOI0.1007/s00253-014-6302-6.4.WenxinLiu,ChaowenYuan,JunBao,WeikunGuan,ZhitengZhao,XingyueLi,JieTang,DandanLi,DongfangShi*.GenerationofanattenuatedstrainoralvaccinecandidateusinganoveldoubleselectionplatforminEscherichiacoli.ApplMicrobiolBiotechnol,2015,99(2):855-867.(3.811),DOI10.1007/s00253-014-6099-3.5.WenxinLiu,ChaowenYuan,XiangqiuMeng,YuanceDu,RuizeGao,JieTang,DongfangShi*.FrequencyofvirulencefactorsinEscherichiacoliisolatedfromsucklingpigswithdiarrhoeainChina.TheVeterinaryJournal,199(2014)286–289.6.YufeiFeng,WenxinLiu,DongfangShi.EffectivenessofEggYolkAntibodyAgainstShigaToxinIIVariantToxicityInVitroandInVivo.CurrentMicrobiology,2013,67(4):448-453.7.KuiyuJiang•YingZhu•WenxinLiu•YufeiFeng•LiliHe•WeikunGuan•WenxiaHu•DongfangShi*.DevelopmentofALoop-MediatedIsothermalAmplification(LAMP)fortheDetectionofF5FimbriaeGeneinEnterotoxigenicEscherichiacoli(ETEC).CurrMicrobiol(2012)65(5):633–638,DOI10.1007/s00284-012-0204-68.杜元策,沙木哈吾·别开,刘文鑫,关玮琨,孟相秋,韩林林,李金萍,袁超文,唐杰,李星月,赵志腾,师东方.重组大肠杆菌肠毒素蛋白LTA_(192)-STa_(13)的表达及其诱导小鼠体液免疫的研究.中国预防兽医学报,2015,(04):304-309.9.韩林林,阿力马斯别克·哈山,李金萍,唐杰,刘文鑫,关玮琨,李星月,赵志腾,师东方.大肠杆菌耐热肠毒素基因多拷贝串联表达及其单克隆抗体的制备.中国预防兽医学报,2015,(03):233-237.10.袁超文,刘文鑫,关玮琨,孟相秋,唐杰,李星月,赵志腾,师东方.牛源产Ⅱ型不耐热肠毒素大肠杆菌的分离鉴定及重组毒素制备[J].畜牧兽医学报,2014,45(8):1336-1341.11.朱颖,刘文鑫,赵姝静,李丹丹,冯瑜菲,关玮琨,何丽丽,江馗语,师东方.稳定表达犬瘟热病毒细胞受体SLAM的BHK-21/SLAM细胞系的建立.中国预防兽医学报,2014,36(6):440-443.12.李丹丹，赵姝静，朱颖，韩林林，孟相秋，李金萍，杜元策，吕广玲，师东方.重组大肠杆菌LTB-Stx2B蛋白的表达及其诱导小鼠体液免疫的研究.中国动物传染病学报，2013,21(5):13-20.13.关玮琨，何丽丽，冯瑜菲，朱颖，刘文鑫，江馗语，胡文霞，师东方.犬细小病毒环介导等温扩增检测方法的建立.中国兽医科学,2013,43(04):407-411.14.何丽丽，关玮琨，江馗语，朱颖，刘文鑫，冯瑜菲，胡文霞，师东方.犬瘟热病毒N基因环介导等温扩增检测方法的建立,中国预防兽医学报,2013,35(2):138~141.15.胡文霞,冯瑜菲,何丽丽,关玮琨,江馗语,师东方.断奶仔猪对毒力基因缺失大肠杆菌的免疫应答[J].中国预防兽医学报,2011,33(6):471-475.